Isolation of Infectious Bronchitis Virus in Primary cells of the Chick Embryo Chorioallantoic Membrane

Adaptation of infectious bronchitis virus in primary cells of the chick embryo chorioallantoic membrane

The susceptibility of the primary chick embryo chorioallontoic membrane cells to infectious bronchitis virus was evaluated after twenty consecutive passages in chick embryo chorioallontoic membrane cells. Virus replication was monitored by cytopathic observation, indirect immunoperoxidase, and reverse transcription polymerase chain reaction (RT-PCR). At 72 hours post-infection (p.i.) in third p...

Evaluating the Effects of Enrofloxacin on Angiogenesis Using the Chick Embryo Chorioallantoic Membrane Model

Objective- Because little information was available in the literature about the angiogenic property of enrofloxacin, this investigation was undertaken to evaluate this aspect using an in vivomodel. Design- Experimental study. Animals- Twenty fertile chicken eggs (Ross 308) with the average egg-weight of 55 ± 0.4 g were rand...

Isolation and Identification of Infectious Bronchitis Virus from Commercial Chickens

Application of the Chick Embryo Chorioallantoic Membrane in Neurosurgery Disease

The chick embryo chorioallantoic membrane (CAM) is a highly vascularized extraembryonic membrane. Because of its ease of accessibility, extensive vascularization and immunodeficient environment, the CAM has been broadly used in the oncology, biology, pharmacy, and tissue regeneration research. The present review summarizes the application of the CAM in neurosurgery disease research. We focused ...

Influenza Virus and Its Mucoprotein Substrate in the Chorioallantoic Membrane of the Chick Embryo

The fate of heat-stable inhibitors (HI) of hemagglutination by influenza viruses in the infected chorioallantoic membrane has been studied. The amount of soluble HI extractable from the normal CAM is taken as a measure of the total mucoprotein substrate derived from the allantoic cells. It has been shown that a decrease in HI is demonstrable when all or nearly all cells of the allantois are inv...